Jinhua is located in the east Jinqu Basin. The study on the isotopic characteristics and renewability of groundwater inthe Jinhua are of important significance for local groundwater resources in the Jinqu Basin. By analyzing the features of spatialdistribution of the stable isotopes deuterium (D) and oxygen-18 (18O) as well as the radioisotopes tritium (3H) and carbon (14C), theprecipitation equation was established: δD=8.29δ18O+15.9. Besides, this paper discussed the isotopic characteristics of the surfacewater, phreatic water and groundwater in the red beds, and evaluated the renewability of phreatic water and groundwater in the redbeds. The results indicate that the slope and intercept of atmospheric precipitation line equation in Jinhua area are greater than theglobal atmospheric precipitation line equation. The hydraulic connection is closely linked among surface water, phreatic water andgroundwater in the red beds. The evaporation process took place after phreatic water and groundwater were recharged by rainfall.The δD and δ18O of the groundwater in the red beds obviously increase in the downstream. The tritium (3H)of the phreatic waterranges from 6.6 to 19.0TU, which shows strong renewability. The 14C age of the groundwater in the red beds ranges from 3020 to5360BP, which shows weak renewability. The tritium (3H) of the groundwater in the red beds ranges from 4.4 to 13.2TU, which showsthat it is mixed with modern water. And δ18O decrease with the age of the groundwater increase.