48 hydrological sites in the Yangtze River Basin were selected to recheck the runoff and design flood. Considering theinfluence of the large reservoirs with strong adjustment abilities in the upstream sites, the reduction calculation and analysis ofrunoff and flood data were carried out to ensure the consistency of the series. Furthermore, a comparative analysis of runoff andflood design results were conducted. Runoff analysis shows that the runoff results of the main control sites in the Yangtze Riverbasin are relatively stable. The change of average annual runoff and 20%, 50% and 75% frequency annual design values of thesites are within 5%. Flood analysis shows that the design flood results with a 100 year return period change within 4% whencomparing with the previous results of different stages.