Tree-ring of picea schrenkiana provides optimum proxies for dendrohydrological research on the northern slope of the western KunlunMountains. The response analysis of tree-ring width standard chronology to the streamflow in the Tizinafu River reveals the significantly positivecorrelation between the ring width index and annual streamflow from August of last year to July of the current year during 1957-2013. The lineartransfer function was established to reconstruct the annual streamflow in the Tizinafu River during 1665-2013. Low frequency characteristics ofthe reconstructed streamflow series show seven drought periods and eight flood periods. The mean value of the reconstructed streamflow is close tothe observed, however, the intensity and frequency of extreme hydrological events of the reconstructed streamflow are higher than the observed.The reconstructed long-term streamflow record provides more reliable reference for the scientific planning and allocation, and the risk evaluationof the downstream reservoirs and other flood-preventing construction projects.