The statistic and anomaly analysis of the runoff characteristic variations in recent 54 years by using the data of the temperature, precipitation and runoff in the Jinghe River mouth from 1959 to 2012. The Mann -Kendall was used to test the natural runoff, and to analyze the factors of precipitation, temperature, population, industry, principal industry. The results show that(1) There are rising trends of the precipitation and temperature changes in the Jinghe River Basin. The runoff is stable, the trendline is almost parallel to X axis of the linear slope of 0.001. (2) The runoff mutated point appears in 1988, passing 0.01significance test, and there is a significant upward trend after 1999. The runoff anomaly change in recent 54 years can be dividedinto two stages: smaller change range from 1958 to 1966, greater change range from 1966 to 2012. (3) The analysis of the impactfactors show that the influence of the human activities on the runoff is great, with contribution rate of 48.98 percent, which ismuch higher than other factors.