The application of distributed hydrological model is usually made depending on the sensitivity analysis and the uncertainty analysis. In order to simulate monthly hydrograph for the Guishuihe River Basin, a distributed hydrological model was developed with SWAT (soil and water assessment tool), calibrated and validated with SUFI -2 (sequential uncertainty fitting program) manually and automatically. The sensitivity analysis was processed by LH-OAT using both SWAT and SUFI-2 and the uncertainty analysis was evaluated by SUFI-2. The criteria of the uncertainty results are P-factor and the R-factor. In this study, R2 =0.59, NSE=0.56 during the calibration period, and R2 =0.82, NSE=0.80 during the validation period. In the both periods, the P-factor is greater than 0.5 and the R-factor is smaller than 0.5.